ClickChemistryTools點擊化學(xué)TAMRA Azide

中文介紹：

紅色熒光 TAMRA 疊氮化物 (TAMRA-PEG4-Azide) 可以通過銅催化的點擊反應(yīng) (CuAAC) 與末端炔烴反應(yīng)。 它還通過無銅“點擊化學(xué)"反應(yīng)與應(yīng)變的環(huán)辛炔反應(yīng)形成穩(wěn)定的三唑，并且不需要銅催化劑或高溫。

TAMRA（四甲基羅丹明）是一種明亮的熒光標(biāo)記，與各種激發(fā)源兼容，包括汞弧、鎢和氙弧燈、氦氖激光器的 544 nm 線和 532 nm 綠色激光線。

英文介紹：

The red-fluorescent TAMRA Azide (TAMRA-PEG4-Azide) can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry" reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

TAMRA (tetramethylrhodamine) is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

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參考文獻(xiàn)：

1. David, L. L., et al. (2021). Chemical Proteomics Approach for Profiling the NAD Interactome. J Am Chem Soc., 143 (18), 6787-6791.