Broadpharm生物素化試劑選擇指南

生物素-親和素相互作用通常用于檢測和/或純化蛋白質(zhì)，因為這兩種分子彼此具有高度特異性。生物素化是將生物素附著在蛋白質(zhì)和其他大分子上的過程。

在選擇生物素化試劑時，用戶需要考慮以下幾個方面。

The biotin–avidin interaction is commonly exploited to detect and/or purify proteins because of the high specificity that these two molecules have for each other. Biotinylation is the process of attaching biotin to proteins and other macromolecules.

When selecting the biotinylating reagents, the user need consider the following aspects.

BroadPharm has all kinds of biotinlyation reagents for meet the need of your advanced research. The variability of these reagents substantially expands the range of applications for avidin–biotin chemistry

• *Varieties of biotin functional group reagents differ in length, solubility, cell permeability and cleavability.

• *Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.

• *Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF.

• *Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable.

BroadPharm擁有各種生物素化試劑，以滿足您高級研究的需求。這些試劑的可變性大大擴展了親和素-生物素化學的應(yīng)用范圍

*各種生物素官能團試劑的長度、溶解度、細胞通透性和裂解性各不相同。

*含有二硫鍵的品種可以使用還原劑進行裂解，使生物素基團能夠與標記的蛋白質(zhì)斷開。

*非磺化NHS-生物素具有細胞滲透性，但必須溶解在有機溶劑中，如DMSO或DMF。

*Sulfo-NHS生物素（以及那些帶有聚乙二醇化間隔物的生物素）是直接水溶性的，但不能滲透膜。