氯膦酸鹽脂質(zhì)體清除肺臟巨噬細(xì)胞全肺流式檢測(cè)解決方案

Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型，全肺臟流式檢測(cè)分群圈門策略，可以參考下圖。Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. 單核巨噬清除訂購(gòu)信息見(jiàn)后。

(A) Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. Interstitial macrophages (IMs, CD45+Siglec-F–CD11b+Ly6G– F4/80+ cells), neutrophils (CD45+Siglec-F–CD11b+Ly6G+ cells), Ly6Chi monocytes (CD45+Siglec-F–CD11b+Ly6G– F4/80– Ly6Chi cells), Ly6Clo monocytes (CD45+Siglec-F–CD11b+Ly6G–F4/80–Ly6Clo cells), CD11b+ DC (CD45+Siglec-F–CD11b+Ly6G– F4/80–Ly6C– CD11c+ MHC-II+cells), CD11b– DCs (CD45+Siglec-F–CD11b– CD3– B220– NK1.1– CD11c+ MHC-II+cells), B cells (CD45+Siglec-F–CD11b– CD3–NK1.1–B220+cells), CD4+ T cells (CD45+Siglec-F–CD11b– CD3+ CD8–CD4+ cells), CD8+ T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8+ cells) and CD4– CD8– T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8– cells).

(B) Absolute numbers of myeloid and lymphoid subsets in the whole lungs of CLL- or PBL-treated control (n=3-4) and Trpv1DTA (n=3) mice after 48h of treatment.

(C) The BALF number of Ly6Chi monocytes, neutrophils, and AMs in CLL- and PBL-treated control (n=4) and Trpv1DTA mice (n=3-4) after 24h of CRKP infection. Statistical analysis: Mann Whitney test (B, left), unpaired ttests (B, right and C to E) with the following significance levels: (*P<0.05, **P<0.01,***P<0.001). ns, not significant.

品牌：Liposoma

產(chǎn)地：荷蘭

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶點(diǎn)科技

部位：肺臟

細(xì)胞：肺泡巨噬細(xì)胞（AM）

劑量：70ul

方式：滴鼻（intratracheal administration）

時(shí)間點(diǎn)：建模前48h

題目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection

期刊：SCIENCE ADVANCES

時(shí)間：6 Sep 2024

期卷：Vol 10, Issue 36

鏈接：DOI: 10.1126/sciadv.adl6162

Liposoma巨噬細(xì)胞清除劑氯膦酸二鈉脂質(zhì)體清除肺泡巨噬細(xì)胞AM：